Southern Police say they have confirmation from Scotland Yard that DNA from a Queenstown girl is not a match for Madeleine McCann.
The Queenstown girl was mistaken for the missing British girl last New Year's Eve.
Police made thorough inquiries into the sighting but were satisfied the girl was not McCann, who went missing from the Ocean Club complex in Praia da Luz, Portugal, in 2007 while on holiday with her parents Kate and Gerry, and siblings Sean and Amelie.
The DNA test result, as requested by Operation Grange - the British police operation investigating Madeleine McCann's disappearance - supports and confirms the original police investigation into the matter.
The sighting is the second of the same girl - the first being in March 2012. At that time Operation Grange did not exist and therefore there was no follow-up request for a DNA test by British authorities.
"[The Queenstown girl's] family has made it very clear that although they appreciate the concerns raised by the public, the family wish to maintain their own and their daughter's privacy," police said.
"Given that there is conclusive evidence that their daughter is not Madeleine McCann and has no connection to Madeleine McCann, it is only right that she and her family are entitled to a level of privacy that most of us enjoy."
Why are fewer teens learning to drive?Related story: Teen non-drivers lazy 'narcissists'