Afghanistan has enough evidence to try only 16 of 88 prisoners that the United States considers a threat to security and plans to free the remaining detainees, the president's spokesman said on Thursday.
The move will further strain relations between the two countries that are already near breaking point over President Hamid Karzai's refusal to sign a security deal to shape the US military presence after most foreign troops leave this year.
Without a deal, Washington could pull most of its troops out after 2014.
The United States is strongly opposed to their release because it says the prisoners, being held in Afghanistan, have been involved in the wounding or killing of US and coalition troops.
State Department spokeswoman Jen Psaki said on Thursday the United States considers 72 of those detainees dangerous.
''These 72 detainees are dangerous criminals against whom there is strong evidence linking them to terror-related crimes, including the use of improvised explosive devices, the largest killer of Afghan civilians,'' Psaki said at a news briefing.
She said ''time will tell'' whether the release of the detainees will affect the signing of the agreement.
Psaki said it was in the interest of the Afghan people and its government to sign it.
The Afghan government says, however, there is no evidence against 45 of the 88 prisoners, while the evidence against a further 27 detainees is not sufficient to put them on trial.
''We cannot allow innocent Afghan citizens to be kept in detention for months and years without a trial for no reason at all,'' Karzai's spokesman, Aimal Faizi, told Reuters.
''We know that unfortunately this has been happening at Bagram, but it is illegal and a violation of Afghan sovereignty and we cannot allow this anymore.''
The president's decision came after the head of Afghanistan's spy agency presented the cases against the prisoners at meeting on Thursday morning.
US senators visiting Afghanistan last week said releasing the prisoners would irreparably damage ties with the United States, but stopped short of saying it would prompt a full military withdrawal.
On Thursday, Senators John McCain and Lindsey Graham said they had made clear to Karzai in Kabul last week their objections to the release, and said there could be some action in response.
''We are in contact with our military and civilian leaders in Afghanistan and will determine what course of action is appropriate once we have received additional information,'' the two Republicans said in a statement.
Karzai has called the so-called ''zero option'' an empty threat and suggested any security deal can wait until after the presidential elections in April.
The United States says it needs time to prepare a post-2014 mission.